Guanidine Thiocyanate (chemical name: Guanidine Thiocyanate, CAS No.: 593-84-0) is a chemical reagent with a clear molecular structure and prominent functional properties. Its molecular formula is C₂H₆N₄S, and its molecular weight is 118.16 g/mol. It appears as an odorless white crystalline powder, which can be easily distinguished from other white powdery reagents in daily observation. The core attribute of this product lies in its "dual functionality"—it not only has excellent hygroscopic ability to actively absorb free moisture in the environment but also can simultaneously exert the effects of protein denaturation and nucleic acid protection in biochemical experimental scenarios. It denatures proteins by disrupting their spatial structure while preventing nucleic acids (DNA/RNA) from being degraded during sample processing. It is a special reagent that meets both industrial moisture-proof needs and scientific research experimental requirements.
The physicochemical properties of Guanidine Thiocyanate provide solid support for its application scenarios, and its core characteristics can be summarized as "three advantages and one note for safety":
At room temperature (20°C), its water solubility can reach 6 M (or 1420 g/L). It dissolves quickly to form a clear and colorless solution, without the need for complex stirring or heating operations. It can be directly used to prepare reagents of the concentration required for experiments, greatly improving the efficiency of sample processing.
Its melting point is stably maintained at 120-122°C (verified by experiments). It is not prone to moisture absorption and caking at room temperature (sealing is required during storage), with strong morphological stability. The remaining reagent after a single use can still maintain a good state.
As a protein denaturant, its mechanism of action is mild and efficient, and it will not damage the structure of nucleic acids. Compared with other denaturants (such as urea), it causes less interference to subsequent nucleic acid detection experiments.
Although its overall chemical properties are stable, it is light-sensitive—long-term exposure to sunlight may affect its purity. In addition, it releases highly toxic gas when in contact with strong acids and is incompatible with strong oxidants and cyanides. These characteristics define clear boundaries for safe operation and help users avoid risks in advance.
To meet the strict requirements of scientific research and industrial scenarios, the key technical parameters of Guanidine Thiocyanate are strictly controlled. The mainstream product specifications are as follows:
- Basic Identification: CAS No. 593-84-0, EC No. 209-812-1, Index No. 615-030-00-5, ensuring product traceability.
- Purity Standard: The purity of research-grade products is ≥98%, with extremely low impurity content, avoiding interference with precise experiments such as nucleic acid extraction.
- Physical Parameters: Density 1.103 g/mL (20°C), refractive index n₂₀/D 1.482, boiling point 132.9°C (under 760 mmHg), flash point 34.2°C, pH value 4.8-6.0 (1420 g/L solution, 20°C). The parameter range is clear, adapting to the adjustment needs of different experimental environments.
- Morphological Specifications: Mostly in the form of crystalline powder. Conventional packaging specifications include 100g/bottle, 500g/bottle, and 1kg/bottle. Users can choose according to their single-use dosage to reduce the risk of reagent deterioration after opening.
The application scenarios of Guanidine Thiocyanate focus on "core scientific research needs" and "auxiliary industrial needs", which can be specifically divided into two fields:
As a key reagent for nucleic acid extraction, it is widely used in the isolation and purification of DNA/RNA from samples such as cells, tissues, and microorganisms:
- Sample Processing Stage: It inactivates and precipitates proteins (such as nucleases and structural proteins) in the sample by denaturing them, preventing nucleic acids from being degraded.
- Adaptable Experimental Scenarios: It is compatible with various sample types such as animal tissues, plant leaves, bacteria, and viruses. It can be used for pre-sample preparation in subsequent experiments such as gene cloning, quantitative real-time PCR (qPCR), gene sequencing, and Western Blot. Currently, 83 relevant literatures (e.g., papers in journals such as *Nucleic Acids Res.* and *Endocrinology*) have verified its reliability in research fields such as gene silencing and neuron isolation.
Relying on its hygroscopicity, it can be used for humidity control in small closed spaces:
- Electronic Component Storage: It is added as a moisture-proof agent to the packaging of electronic components (such as chips and resistors) to absorb residual moisture in the packaging and prevent short circuits caused by component moisture.
- Precision Instrument Transportation: During the transportation of optical instruments and medical equipment, it is used in conjunction with desiccants to temporarily maintain a low-humidity environment inside the packaging and protect the precision components of the instruments.
To ensure transportation safety and product quality, the transportation of Guanidine Thiocyanate must strictly comply with international dangerous goods transportation standards. The specific requirements are as follows:
- Transportation Identification: UN Dangerous Goods Number 1759 (universal for European land transportation, international maritime transportation, and international air transportation), classified as Class 8 Corrosive Solids, Packing Group III (slightly hazardous). The transportation name is marked as "CORROSIVE SOLID, N.O.S. (Guanidinium Thiocyanate)", and GHS hazard symbols (GHS05 for Corrosion, GHS07 for Harmful) must be affixed to the outer packaging.
- Mixed Transportation Taboos: It is strictly prohibited to transport it together with strong acids, strong oxidants, or cyanides to avoid the release of highly toxic gases or chemical reactions due to reagent contact.
- Packaging Protection: Double-layer sealed packaging (inner polyethylene plastic bag + outer paper/metal can) is adopted to prevent reagent leakage caused by packaging damage during transportation. Desiccants must be placed inside the can to prevent moisture intrusion.
- Environmental Control: During transportation, direct sunlight must be avoided (to prevent deterioration due to light sensitivity), and the temperature should be controlled within 5-30°C (room temperature range). Rain or soaking is prohibited.
- Emergency Preparation: Transportation vehicles must be equipped with dry powder fire extinguishers (water-containing fire extinguishers are prohibited for extinguishing this product), and a Chinese version of the Material Safety Data Sheet (MSDS) must be carried with the vehicle to facilitate the rapid adoption of first-aid measures in emergency situations such as leakage and combustion.to eyes, respiratory system and skin.Causes burns
Guanidine Thiocyanate (chemical name: Guanidine Thiocyanate, CAS No.: 593-84-0) is a chemical reagent with a clear molecular structure and prominent functional properties. Its molecular formula is C₂H₆N₄S, and its molecular weight is 118.16 g/mol. It appears as an odorless white crystalline powder, which can be easily distinguished from other white powdery reagents in daily observation. The core attribute of this product lies in its "dual functionality"—it not only has excellent hygroscopic ability to actively absorb free moisture in the environment but also can simultaneously exert the effects of protein denaturation and nucleic acid protection in biochemical experimental scenarios. It denatures proteins by disrupting their spatial structure while preventing nucleic acids (DNA/RNA) from being degraded during sample processing. It is a special reagent that meets both industrial moisture-proof needs and scientific research experimental requirements.
The physicochemical properties of Guanidine Thiocyanate provide solid support for its application scenarios, and its core characteristics can be summarized as "three advantages and one note for safety":
At room temperature (20°C), its water solubility can reach 6 M (or 1420 g/L). It dissolves quickly to form a clear and colorless solution, without the need for complex stirring or heating operations. It can be directly used to prepare reagents of the concentration required for experiments, greatly improving the efficiency of sample processing.
Its melting point is stably maintained at 120-122°C (verified by experiments). It is not prone to moisture absorption and caking at room temperature (sealing is required during storage), with strong morphological stability. The remaining reagent after a single use can still maintain a good state.
As a protein denaturant, its mechanism of action is mild and efficient, and it will not damage the structure of nucleic acids. Compared with other denaturants (such as urea), it causes less interference to subsequent nucleic acid detection experiments.
Although its overall chemical properties are stable, it is light-sensitive—long-term exposure to sunlight may affect its purity. In addition, it releases highly toxic gas when in contact with strong acids and is incompatible with strong oxidants and cyanides. These characteristics define clear boundaries for safe operation and help users avoid risks in advance.
To meet the strict requirements of scientific research and industrial scenarios, the key technical parameters of Guanidine Thiocyanate are strictly controlled. The mainstream product specifications are as follows:
- Basic Identification: CAS No. 593-84-0, EC No. 209-812-1, Index No. 615-030-00-5, ensuring product traceability.
- Purity Standard: The purity of research-grade products is ≥98%, with extremely low impurity content, avoiding interference with precise experiments such as nucleic acid extraction.
- Physical Parameters: Density 1.103 g/mL (20°C), refractive index n₂₀/D 1.482, boiling point 132.9°C (under 760 mmHg), flash point 34.2°C, pH value 4.8-6.0 (1420 g/L solution, 20°C). The parameter range is clear, adapting to the adjustment needs of different experimental environments.
- Morphological Specifications: Mostly in the form of crystalline powder. Conventional packaging specifications include 100g/bottle, 500g/bottle, and 1kg/bottle. Users can choose according to their single-use dosage to reduce the risk of reagent deterioration after opening.
The application scenarios of Guanidine Thiocyanate focus on "core scientific research needs" and "auxiliary industrial needs", which can be specifically divided into two fields:
As a key reagent for nucleic acid extraction, it is widely used in the isolation and purification of DNA/RNA from samples such as cells, tissues, and microorganisms:
- Sample Processing Stage: It inactivates and precipitates proteins (such as nucleases and structural proteins) in the sample by denaturing them, preventing nucleic acids from being degraded.
- Adaptable Experimental Scenarios: It is compatible with various sample types such as animal tissues, plant leaves, bacteria, and viruses. It can be used for pre-sample preparation in subsequent experiments such as gene cloning, quantitative real-time PCR (qPCR), gene sequencing, and Western Blot. Currently, 83 relevant literatures (e.g., papers in journals such as *Nucleic Acids Res.* and *Endocrinology*) have verified its reliability in research fields such as gene silencing and neuron isolation.
Relying on its hygroscopicity, it can be used for humidity control in small closed spaces:
- Electronic Component Storage: It is added as a moisture-proof agent to the packaging of electronic components (such as chips and resistors) to absorb residual moisture in the packaging and prevent short circuits caused by component moisture.
- Precision Instrument Transportation: During the transportation of optical instruments and medical equipment, it is used in conjunction with desiccants to temporarily maintain a low-humidity environment inside the packaging and protect the precision components of the instruments.
To ensure transportation safety and product quality, the transportation of Guanidine Thiocyanate must strictly comply with international dangerous goods transportation standards. The specific requirements are as follows:
- Transportation Identification: UN Dangerous Goods Number 1759 (universal for European land transportation, international maritime transportation, and international air transportation), classified as Class 8 Corrosive Solids, Packing Group III (slightly hazardous). The transportation name is marked as "CORROSIVE SOLID, N.O.S. (Guanidinium Thiocyanate)", and GHS hazard symbols (GHS05 for Corrosion, GHS07 for Harmful) must be affixed to the outer packaging.
- Mixed Transportation Taboos: It is strictly prohibited to transport it together with strong acids, strong oxidants, or cyanides to avoid the release of highly toxic gases or chemical reactions due to reagent contact.
- Packaging Protection: Double-layer sealed packaging (inner polyethylene plastic bag + outer paper/metal can) is adopted to prevent reagent leakage caused by packaging damage during transportation. Desiccants must be placed inside the can to prevent moisture intrusion.
- Environmental Control: During transportation, direct sunlight must be avoided (to prevent deterioration due to light sensitivity), and the temperature should be controlled within 5-30°C (room temperature range). Rain or soaking is prohibited.
- Emergency Preparation: Transportation vehicles must be equipped with dry powder fire extinguishers (water-containing fire extinguishers are prohibited for extinguishing this product), and a Chinese version of the Material Safety Data Sheet (MSDS) must be carried with the vehicle to facilitate the rapid adoption of first-aid measures in emergency situations such as leakage and combustion.to eyes, respiratory system and skin.Causes burns
